News — Western Blot

Need Help Deciding Which Membrane Material to Use for Your Western Blots?

Posted by BD TEAM on

Purchase Transfer Membranes at MSE Supplies The choice of membrane for Western Blotting depends on the specific requirements of the experiment and the target protein being analyzed. Each membrane material has its own characteristics and advantages. Here's a comparison of PVDF (polyvinylidene difluoride), nitrocellulose, and nylon membranes commonly used in Western blotting: PVDF Membrane: Advantages: PVDF membranes have high protein-binding capacity, making them suitable for low-abundance protein detection. They are durable, chemically resistant, and compatible with a wide range of antibody incubation conditions. PVDF membranes also have low background noise and offer excellent sensitivity. Considerations: PVDF membranes require pre-wetting with...

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