MSE PRO™ Horse ADM(Adrenomedullin) ELISA Kit

Product name	Horse ADM(Adrenomedullin) ELISA Kit
Reactivity	Horse
Alternative Names	AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
Assay Type	Sandwich
Sensitivity	5.02 pg/mL
Standard	1000 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Type	serum, plasma and other biological fluids
Assay Length	3.5h
Research Area	Endocrinology; Hormone metabolism
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ADM in the samples is then determined by comparing the OD of the samples to the standard curve.

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