Accuris qMAX One-Step RT-qPCR Kits

RNA to cDNA to qPCR, in one tube
High purity enzyme formulation for enhanced stability and performance
Accuris Hot-Start Taq allows for preparation at room-temperature
Blue dye facilitates pipetting and visualization in plates
Available for green fluorescence or probe detection
Multiplex formulation available for multiple target amplification
Bulk pricing available for high throughput labs and kit manufacturers contact us for details
Check out our One Step Technical Bulletin

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Bulk Packaging Available

To accommodate the requirements of high throughput users, Accuris is now offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Purchase Accuris qPCR Bulk Reagent Distributor Pricing through MSE Supplies Here!

Model

SKU	Description
PR2120-L-100	Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 reactions
PR2120-L-500	Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 500 reactions
PR2120-L-1000	Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 reactions
PR2120-H-100	Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 100 reactions
PR2120-H-500	Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 500 reactions
PR2120-H-1000	Accuris qMAX Green One-Step RT-qPCR Kit, High Rox, 1000 reactions
PR2121-N-100	Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 100 reactions
PR2121-N-500	Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 500 reactions
PR2121-N-1000	Accuris qMAX Probe One-Step RT-qPCR Kit, No Rox, 1000 reactions
PR2121-L-100	Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions
PR2121-L-500	Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 500 reactions
PR2121-L-1000	Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 1000 reactions
PR2121-H-100	Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 100 reactions
PR2121-H-500	Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 500 reactions
PR2121-H-1000	Accuris qMAX Probe One-Step RT-qPCR Kit, High Rox, 1000 reactions
PR2122-N-100	Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 100 reactions
PR2122-N-500	Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 500 reactions
PR2122-N-1000	Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, No Rox, 1000 reactions

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