Recombinant Rat IL-1A/ IL-1α Protein
SKU: PKSR030449-50
Recombinant Rat IL-1A/ IL-1α Protein
| SKU # | PKSR030449 |
| Expression Host | E.coli |
Description
| Synonyms | interleukin, Hematopoietin, IL1F1hematopoietin, IL1F, IL-1F, alpha, BAF, Hematopoietin-1, IL1, IL1 alpha, IL-1 alpha, IL1-A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, IL1F1hematopoietin-1, interleukin 1, interleukin-1 alpha, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha, Il1a, FAF, Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL1 ALPHA, IL1F1, Interleukin 1 alpha, LAF, LEM, Preinterleukin 1 alpha, Il1a |
| Species | Rat |
| Expression Host | E.coli |
| Sequence | Ser115-Ser270 |
| Accession | P16598 |
| Calculated Molecular Weight | 17.9 kDa |
| Observed Molecular Weight | 16 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin 1 (IL-1) is a name that designates two proteins, IL-1αand IL-1β, which are the products of distinct genes, but which show approximately 25% amino acid (aa) sequence identity and which recognize the same cell surface receptors. IL-1αand IL-1β are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1α remains in the cytosol of cells, although there is evidence for a membranebound form of the precursor form of IL-1α. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen.