Recombinant Mouse Interleukin-22/IL-22 Protein (Active)
SKU: PKSM041085-100
Recombinant Mouse Interleukin-22/IL-22 Protein (Active)
| SKU # | PKSM041085 |
| Expression Host | E.coli |
Description
| Synonyms | Interleukin, Cytokine Zcyto, IL-D, TIFIL, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22a, IL-D110, IL-TIF, Interleukin-22a, TIFa, TIFIL-23, UNQ3099/PRO10096, zcyto18, Interleukin-22, T-cell-derived-inducible, factor(IL-TIF), Interleukin-22a(IL-22a), IL-TIF alpha, IL-10-related, IL-22, Il22a, Iltif, Iltifa, Il22, TIFa |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Leu34-Val179 |
| Accession | Q9JJY9 |
| Calculated Molecular Weight | 17.6 kDa |
| Observed Molecular Weight | 17 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce IL-10 secretion in COLO205 cells. The ED50 for this effect is <0.3 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature protein that shares approximately 79% and 22% sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.