Recombinant Mouse DDR1 Kinase/MCK10 Protein (His &GST Tag)(Active)
SKU: PKSM040294-50
Recombinant Mouse DDR1 Kinase/MCK10 Protein (His &GST Tag)(Active)
| SKU # | PKSM040294 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | 6030432F18, AI323681, CD167a, Cak, Nep, PTK3A |
| Species | Mouse |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Leu444-Val874 |
| Accession | Q03146-2 |
| Calculated Molecular Weight | 75.8 kDa |
| Observed Molecular Weight | 68 kDa |
| Tag | N-His-GST |
| Bio-activity | The specific activity was determined to be 2 nmol/min/mg using synthetic modified AXLtide peptide (modified-CKKSRGDYMTMQIG) as substrate. |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM DTT |
| Reconstitution | Not Applicable |
Background
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.