Recombinant Human BMP-11 protein(N-His)(active)
SKU: PKSH034138-100
Recombinant Human BMP-11 protein(N-His)(active)
| SKU # | PKSH034138 |
| Expression Host | E.coli |
Description
| Synonyms | GDF-11, Growth/Differentiation Factor-11 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Asn 299-Ser 407 |
| Accession | O95390 |
| Calculated Molecular Weight | 13.4 kDa |
| Observed Molecular Weight | 16 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED50 for this effect is <11 ng/mL.Measure by its ability to induce hemoglobin expression in K562 cells. The ED50 for this effect is <4 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues. It is required for proper vertebral patterning and orofacial development. Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3.