Reactive Oxygen Species (ROS) Fluorometric Assay Kit
SKU: E-BC-K138-F-96
Reactive Oxygen Species (ROS) Fluorometric Assay Kit
| SKU # | E-BC-K138-F |
| Detection Instrument | Fluorescence Microplate reader / Fluorescence Spectrophotometer / Flow Cytometry(Ex/Em=500 nm/525 nm) |
| Detection method | Fluorescence method |
Product Details
Properties
| Synonyms | ROS, DCFH-DA, DCFH, DCF |
| Sample Type | Fresh tissue, cultured cells |
| Detection Method | Fluorescence method |
| Assay type | Cell-based (quantitative) |
| Assay time | 60 min |
| Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Storage | Reagent 1: -20℃, Reagent 2: 2-8℃ |
| Valid period | 12 months |
Images
YF Yang et al investigate the function of Danshen Dripping Pill in inhibiting heart failure. Reactive oxygen species (ROS) in mouse heart was determined using ROS fluorometric assay kit (E-BC-K138-F).
ROS level in mouse heart was significantly increased in Ctrl group and LST group, and reversed in HST, CDDP and CST groups. (***P<0.001, ###P<0.001)
Detection Principle
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Kit Components & Storage
| Item | Component | Size 2(96 T) | Storage |
| Reagent 1 | 10 mmol/L DCFH-DA | 0.1 mL × 1 vial | -20°C, 12 months, shading light |
| Reagent 2 | Positive Control | 1 mL ×1 vial | 2-8°C, 12 months |
| Black Microplate | 96 wells | No requirement | |
| Plate Sealer | 4 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.