PRKAR2A Polyclonal Antibody
SKU: E-AB-52196-200
PRKAR2A Polyclonal Antibody
| SKU # | E-AB-52196 |
| Reactivity |
Human |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human PRKAR2A |
| Abbre | PRKAR2A |
| Synonyms | cAMP dependent protein kinase regulatory subunit RII alpha, cAMP dependent protein kinase regulatory subunit alpha 2, cAMP dependent protein kinase type II alpha regulatory chain, cAMP dependent protein kinase type II alpha regulatory subunit, cAMP-dependent |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane. |
| Concentration | 0.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:35-1:200 |
Background
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).