Phospho-PI 3 Kinase p85 Alpha /Gamma (Tyr467/199) Polyclonal Antibody
SKU: E-AB-20966-200
Phospho-PI 3 Kinase p85 Alpha /Gamma (Tyr467/199) Polyclonal Antibody
| SKU # | E-AB-20966 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Tyr467/199 |
| Synonyms | GRB1, P85A, Phosphatidylinositol 3 kinase, Phosphatidylinositol 3 kinase associated p 85 alpha, Phosphatidylinositol 3 kinase regulatory 1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, p85, p85 alpha, polypeptide 1 (p85 alpha), regulatory subunit |
| Swissprot | |
| Calculated MW | 54 +83kDa |
| Observed MW | 55+85 kDa |
| Cellular Localization | Cytoplasmic |
| Tissue Specificity | PI 3 kinase p85 alpha is Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).1 PublicationPI 3 kinase p85 gamma isHighest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.