PCNA Monoclonal Antibody Reactivity Zebrafish
SKU: E-AB-48013-200
PCNA Monoclonal Antibody Reactivity Zebrafish
| SKU # | E-AB-48013 |
| Reactivity | Zebrafish |
| Host | Mouse |
| Applications | WB, IF, IHC |
Product Details
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Zebrafish |
| Applications | WB, IF, IHC |
| Clonality | Monoclonal |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Abbre | PCNA |
| Synonyms | Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNAR, Pcna/cyclin, Polymerase delta accessory protein, Proliferating ce, cb16, etID36690.10, fa28e03, fb36g03, ATLD2 |
| Swissprot | |
| Calculated MW | 30 kDa |
| Observed MW | 36 kDa |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers |
| Clone No. | 8B12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-2000 |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.