GSK3 alpha/beta Polyclonal Antibody
SKU: E-AB-31628-200
GSK3 alpha/beta Polyclonal Antibody
| SKU # | E-AB-31628 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human GSK3α/β around the non-phosphorylation site of Tyr279/216. |
| Abbre | GSK3 alpha/beta |
| Synonyms | GSK-3 alpha, GSK-3 beta, GSK3A, GSK3B, Glycogen synthase kinase-3 alpha, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3A, Serine/threonine-protein kinase GSK3B |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW | 51 ,46kDa |
| Cellular Localization | Cytoplasmic and Nuclear. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3βGSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.