GCLC Polyclonal Antibody
SKU: E-AB-52359-200
GCLC Polyclonal Antibody
| SKU # | E-AB-52359 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human GCLC |
| Abbre | GCLC |
| Synonyms | EC 6.3.2.2, GCL, GCS, GCS heavy chain, GLCL, GLCLC, GSH1, Gamma ECS, Gamma glutamylcysteine synthetase, Gamma-ECS, Gamma-glutamylcysteine synthetase, Gclc, Glutamate cysteine ligase catalytic subunit, Glutamate--cysteine ligase catalytic subunit |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytosol, Other locations: glutamate-cysteine ligase complex. |
| Concentration | 0.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:25-1:100 |
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.