E-Cadherin Polyclonal Antibody Store at -20°C
SKU: E-AB-31261-200
E-Cadherin Polyclonal Antibody Store at -20°C
| SKU # | E-AB-31261 |
| Reactivity | Human |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the N-terminal region of human E-cadherin |
| Abbre | E-Cadherin |
| Synonyms | Arc 1, CADH1, CAM 120/80, CD 324, CD324, CD324 antigen, CDHE, Cadherin 1, Cadherin1, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin, cadherin 1 type 1 E-cadherin, cdh1, epithelial calcium dependant adhesion protein |
| Swissprot | |
| Calculated MW | 97 kDa |
| Observed MW | 100 kDa |
| Cellular Localization | Cell junction. Cell membrane. Endosome. Golgi apparatus>trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Developmental Biology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.