CCDC99 Polyclonal Antibody Store at -20°C
SKU: E-AB-10614-200
CCDC99 Polyclonal Antibody Store at -20°C
| SKU # | E-AB-10614 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein of human SPDL1 |
| Abbre | SPDL1 |
| Synonyms | AA409762, Arsenite related gene 1 protein, Arsenite-related gene 1 protein, CCDC 99, Ccdc99, Coiled coil domain containing 99, Coiled-coil domain-containing protein 99, FLJ20364, FLJ40690, MGC124977, Protein Spindly, Rh, hSpindly, rhabdomyosarcoma antigen MU RMS 40.4A |
| Swissprot | |
| Calculated MW | 70/59 kDa |
| Cellular Localization | Cytoplasm>cytoskeleton>centrosome. Chromosome>centromere>kinetochore. Nucleus. Cytoplasm>cytoskeleton>spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex. |
| Concentration | 0.2 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
Background
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect.