CA9 Monoclonal Antibody
SKU: E-AB-22034-200
CA9 Monoclonal Antibody
| SKU # | E-AB-22034 |
| Reactivity | Human |
| Host | Mouse |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | CA IX |
| Synonyms | CA-IX, CA9, CAH9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, MN, Membrane antigen MN, P54/58N, RCC associated protein G250, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250, pMW1 |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW | 35-38 kDa |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| Tissue Specificity | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Signal Transduction |
| Clone No. | 9B5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-1:3000 |
| IHC | 1:50-300 |
| IF | 1:50-1:200 |
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.