BRCA1 Polyclonal Antibody Store at -20°C Host Rabbit
SKU: E-AB-30669-200
BRCA1 Polyclonal Antibody Store at -20°C Host Rabbit
| SKU # | E-AB-30669 |
| Reactivity | Human, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p, IF |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of Ser1423. |
| Abbre | BRCA1 |
| Synonyms | BRCA 1, BRCA1, BRCA1 DNA repair associated, BRCA1/BRCA2 containing complex subunit 1, BRCA1/BRCA2-containing complex, BRCAI, BRCC 1, BRCC1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast and ovarian cancer susceptibility protein 1, Breast cance, subunit 1 |
| Swissprot | |
| Calculated MW | 208 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
| IF | 1:200-1:1000 |
Background
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.