Bioneer RocketScript Reverse Transcriptase (50,000 Units)
SKU: E-3142
Bioneer RocketScript™ Reverse Transcriptase (50,000 Units)
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RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistance to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.
※This product is shipped in dry ice.
Features and Benefits
Thermostability
Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
User-friendly
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
Reproducibility
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
Applications
- Gene synthesis
- First-strand synthesis of cDNA from RNA molecules (reverse transcription)
- RT-PCR
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5'-end mapping by primer extension analysis
- Real time PCR
Specifications
| 5' to 3' exonuclease | No |
| 3' to 5' exonuclease | No |
| 3' – A overhang | No |
| Fragment size | Up to 10 kb |
Components
5x Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.5)
100 mM DTT
10 mM dNTPs mix: 2.5 mM of each dNTP
Concentration
10,000 Units (200 U/μl)
Storage Conditions
50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers.
Storage Temperature
-20°C