ATTO AB-2550 Kronos Dio-Real Time Monitoring Gene Expression in Cultured Cells and Tissue Slices
SKU: AB-2550
ATTO AB-2550 Kronos Dio-Real Time Monitoring Gene Expression in Cultured Cells and Tissue Slices
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AB-2550 Chronos Dio is a luminometer that uses a photomultiplier tube as a detector. It is ideal for monitoring gene expression in cultured cells, tissue slices, etc. in real time for several hours to several days using a 35mm diameter culture dish as a sample container.
Purpose and Application
- Luciferase reporter assay for real-time gene expression (transcriptional activity) in live cells
-
Response of cultured cells to drug stimulation
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Technical Data:
| Model | AB-2550 |
|---|---|
|
Measuring vessel |
Cell culture dish 35mm diameter |
|
Number of specimens |
8 pieces (8 dishes can be set) |
|
Constant temperature method |
Peltier/Air Circulation Method |
|
Chamber temperature setting |
(Room temperature -5℃) to 45℃ in 1℃ increments |
|
Temperature accuracy inside the chamber |
±0.5℃ (at room temperature of 25℃) |
|
CO2 gas |
Controlled by sensor and solenoid valve (concentration 5%) |
|
CO2 Gas Sensor Accuracy |
±0.1% |
|
Detector |
Photomultiplier tube |
|
Measurement method |
Photon counting method using photomultiplier tubes |
|
Detection Wavelength Range |
350nm to 670nm |
|
Luminescence measurement time |
1 to 60 seconds/1 to 60 minutes |
|
Filters |
F0: None (all light) F1: O56 filter F2: R62 filter |
|
Color separation mechanism |
Automatic filter switching mechanism allows separation of up to 3 colors |
|
control |
Control software from a PC (Windows 11/10) (up to 5 units can be controlled individually) |
|
Connecting to a PC |
USB |
|
Dimensions(mm) |
280(W)×400(D)×330(H) |
|
Weight(Kg) |
16 |
|
power supply |
AC100-230V, 50/60Hz, 110W |
Citation and References
-
(Anti-inflammation, IL-6, IL-10) P. Saiki, Y. Nakajima, LJLD. Van Griensven, K. Miyazaki, Real-time monitoring of IL-6 and IL-10 reporter expression for anti-inflammation activity in live RAW 264.7 cells. Biochem. Biophys. Res. Commun., 503(3), 885-890 (2018)[PubMed]
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(Clock gene, UV stress) G. Kawamura, M. Hattori, K. Takamatsu, T. Tsukada, Y. Ninomiya, I. Benjamin, P. Sassone-Corsi, T. Ozawa and T. Tamaru, Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress. Commun. Biol., 1, 204 (2018)[PubMed]
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(Clock gene) AR. Saran, D. Kalinowska, S. Oh, R. Janknecht, L. DiTacchio, JMJD5 links CRY1 function and proteasomal degradation. PLoS Biol., 16(11), e2006145 (2018)[PubMed]
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(Clock gene) J. Lee, E. Park, GH. Kim, I. Kwon and K. Kim, A splice variant of human Bmal1 acts as a negative regulator of the molecular circadian clock. Exp. Mol. Med., 50(12), 159 (2018)[PudMed]
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(Clock gene) Y. Maruyama, Y. Asaoka, K. Nakahama, T. Tamaru, K. Takamatsu, N. Takamatsu, A. Hattori, S. Nishina, N. Azuma, A. Kawahara, K. Kume and H. Nishina, The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish. Sci. Rep., 9(1), 196 (2019)[PubMed]
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(Clock gene, ER stress) L. Gao, H. Chen, C. Li, Y. Xiao, D. Yang, M. Zhang, D. Zhou, W. Liu, A. Wang and Y. Jin, ER stress activation impairs the expression of circadian clock and clock-controlled genes in NIH3T3 cells via an ATF4-dependent mechanism. Cell. Signal., 57, 89-101 (2019)[PubMed]