Alpha Tubulin Monoclonal Antibody
SKU: E-AB-20036-200
Alpha Tubulin Monoclonal Antibody
| SKU # | E-AB-20036 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Applications | WB, IHC-p, IF, IP |
Product Details
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Protein |
| Abbre | α-tubulin |
| Synonyms | Alpha-tubulin 3, Alpha-tubulin ubiquitous, TUBA1A, TUBA1B, TUBA3, Tubulin B-alpha-1, Tubulin K-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain |
| Swissprot |
Q71U36P68363
|
| Calculated MW | 50 kDa |
| Observed MW | 52 kDa |
| Cellular Localization | Cytoskeleton, cytoplasmic microtubule, microtubule, microtubule cytoskeleton, Cytosol, Endosome, recycling endosome, Extracellular region or secreted, extracellular exosome, Nucleus, Other locations: cytoplasmic ribonucleoprotein granule, membrane raft, myelin sheath. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Signal Transduction |
| Clone No. | 5M5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-10000 |
| IHC | 1:50-300 |
| IF | 1:50-1:200 |
| IP | 1:100-1:300 |
Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.