0.25% Trypsin Solution, 100mL

Summary

Trypsin is a proteolytic enzyme that's commonly used to detach adherent cells from culture surfaces in cell culture. It's a standard method for cell dissociation and is used for a variety of purposes, including:

• Cell harvesting
  Trypsin breaks down proteins that adhere cells to vessels, making them easier to resuspend
• Subculture
  Trypsin is used to detach cells from the substrate so they can be subdivided and reseeded into fresh cultures
• Cell counting
  Trypsin is used to create cell suspensions that can be counted for analysis