SARS-CoV-2 Neutralization Antibody ELISA Kit
SARS-CoV-2 Neutralization Antibody ELISA Kit
| Detection Range | 15.63-500 ng/mL |
| Sensitivity | 9.38 ng/mL |
Product Details
Properties
| Assay Type | One step Competitive-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Sample type | serum and plasma |
| Sample volume | 50 μL |
| Storage | 2-8℃ |
| Interpretation | Semi-Quantitative |
| Application | This ELISA kit applies to the in vitro Semi-Quantitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma. |
| Reproducibility | Both intra-CV and inter-CV are < 10% |
| Synonyms | COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody |
Test Principle
This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.
Kit Components and Storage
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the
reagents according to the conditions on the next page.
| Item | Specifications | Storage |
| Micro ELISA Plate(Dismountable) |
24T: 8 wells ×3 strips 96T: 8 wells ×12 strips |
2-8℃, 1 week |
| Reference Standard | 24T: 1 vial | 2-8℃, 6 months |
| 96T: 2 vials | ||
| Positive Control | 24T: 1 vial | |
| 96T: 2 vials | ||
| Negative Control | 24T: 1 vial | |
| 96T: 2 vials | ||
| Concentrated HRP Conjugated RBD (HRP-RBD, 100×) |
24T: 1 vial, 60 μL | 2-8℃(Protect from light), 6 months |
| 96T: 1 vial, 120 μL | ||
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| HRP Conjugated RBD Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer(25×) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃(Protect from light) , 6 months |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and
high level SARS -CoV-2 Nab were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and
high level SARS -CoV-2 Nab were tested on 3 different plates, 20 replicates in each
plate, respectively
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 17.74 | 110.98 | 413.33 | 18.21 | 117.00 | 424.56 |
| Standard deviation | 0.84 | 7.41 | 34.77 | 1.28 | 9.77 | 28.65 |
| CV (%) | 4.73 | 6.68 | 8.41 | 7.05 | 8.35 |
6.75 |
Recovery
The recovery of SARS-CoV-2 Nab spiked at three different levels in samples
throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-101 | 94 |
| EDTA plasma(n=8) | 95-107 | 101 |
| Cell culture media(n=8) | 92-106 | 98 |
Assay Procedures
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1. Add 50µL each pre-treated Samples and Controls. Immediately add 50µL of HRP-RBD working solution. Incubate for 60 min at 37°C. |
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2. Aspirate and wash the plate for 3 times. |
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3. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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4. Add 50µL of Stop Solution. |
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5. Read the plate at 450nm immediately. Calculation of the results |