MSE PRO Cell Fusion System for Electrofusion/Electroporation
MSE PRO™ Cell Fusion System for Electrofusion/Electroporation
The MSE PRO™ Cell Fusion System utilizes pulses of electric fields that can induce the fusion of neighboring cells (electrofusion) or create a porous cell membrane (electroporation) to allow for transfection of macromolecules. Both techniques are reproducible and non-toxic techniques widely used in researching cell-cell interactions and intracellular pathways. Many applications have clinical significance in diagnostic testing, therapeutics and vaccine development.
Offered in 4 different electrode spacings and volume inputs
- SKU# MA1005: 0.5mm Electrode Spacing, 20μL volume input
- SKU# MA1006: 1.0mm Electrode Spacing, 40μL volume input
- SKU# MA1007: 2.0mm Electrode Spacing, 80μL volume input
- SKU# MA1008: 3.2mm Electrode Spacing, 700μL volume input
Applications
- Stem cell development
- Nuclear transfer for transgenic development
- Production of hybridomas (fusion of B-cells and Myeloma cells) for antibody expression
- Genetically modified plant protoplast, tetraploid fusion
- Embryo manipulation
- Live gene/drug introduction
Specifications
| SKU # | MA1005 | MA1006 | MA1007 | MA1008 |
| Electrode Spacing (mm) | 0.5 | 1.0 | 2.0 | 3.2 |
| Sample Input Volume (μL) | 20 | 40 | 80 | 700 |
| Dimensions (LxWxH, mm) | 450x370x290 | 450x370x290 | 450x370x290 | 450x370x290 |
| Power Source (V/Hz) | 110/60 | 110/60 | 110/60 | 110/60 |
Performance Descriptions
For maintenance of the machine, simply clean frequently using a mild cleaning agent and then disinfect with Ethanol.
| Performance | Description |
| String AC electric field frequency 1MHz-2.5MHz (optimal stable value) | Biological cells can be arranged in strings in an alternating electric field and come into contact with each other. This frequency is designed for a wide range to provide the best frequency choice for the arrangement and fusion of different types of cells or protoplasts of animals, plants, and microorganisms |
| String AC electric field voltage 1-58V | This voltage range can collect cells ranging from plant protoplasts to bacterial volumes. This voltage range also meets the voltage needs of large volume fusion chambers with wide electrode spacing. |
| Fusion pulse voltage range 5-600V | This voltage range allows biological cells of any size to induce confluence in fusion chambers at different pole distances. |
| Gate time 10-1000US | The AC field can be turned off at a set time when a fusion pulse is applied. This is equivalent to inserting a DC pulse into the AC pulse. |
| Pulse width 5-5000US | Precisely control the timing of fusion pulses. Inducing fusion with multiple short pulses yields higher cell viability than confluence with a single long pulse. |
| Pulses 1-9 | Adjusting the number of fusion pulses applied adds a selection index to determine the optimal fusion conditions for biological cells. |
Alternating Electrical Field Parameters
| Square Wave Series Pulse Voltage | Peak-to-peak 1-58V, continuously adjustable |
| Square Wave Series Pulse Frequency | 30KH-3000KHz, continuously adjustable |
| Gate time | 10-1000μs, adjustable in 1μs increments, continuously adjustable |
Direct Current Electrical Field Parameters
| Square Wave Fusion Pulse Voltage | 5-600V, continuously adjustable, field strength up to 6000V/cm with electrode spacing of 1.0mm |
| Square Wave Fusion Pulse Width | 5-600V, continuously adjustable, field strength up to 6000V/cm with electrode spacing of 1.0mm |
| Square Wave Fusion Pulse Cycles | 1-9 |
| Randomly Matched Plate Electrodes: | Electrode spacing of 1mm, 2mm |
| Display Mode | 7-inch TFT Touchscreen |
| Optional Electrodes | Microscope Slide |