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Mouse BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit

SKU: E-EL-M2411

  • £40700
  • Save £7300


Mouse BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit

Detection Range 0.03-20 ng/mL
Sensitivity 0.02 ng/mL



Product Details

Properties

Format 96T
Assay time 3.5h
Sample type serum, plasma and other biological fluids
Sample volume 100μL
Sample size Run up to 46 samples in duplicate and 30 samples in triplicate
Storage 2-8℃
Interpretation Detection range: 0.03-20 ng/mL
Sensitivity: 0.02 ng/mL
Application Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms B2MG, B2M

 

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMG/β2-MG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMG/β2-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMG/β2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BMG/β2-MG. You can calculate the concentration of Mouse BMG/β2-MG in the samples by comparing the OD of the samples to the standard curve.

 

Kit Components and Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

 

 

Technical Data:

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and
high level Mouse BMG/β2-MG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and
high level Mouse BMG/β2-MG were tested on 3 different plates, 20 replicates in each
plate, respectively.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.9 1.8 8.2 0.9 1.7 8.8
Standard deviation 0.1 0.1 0.3 0.1 0.1 0.4
CV (%) 11.11 5.56 3.66 11.11 5.88 4.55

 

 Recovery

The recovery of Mouse BMG/β2-MG spiked at three different levels in samples
throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 96
EDTA plasma (n=8) 86-100 93
Cell culture media (n=8) 84-96 90

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results