Bovine TG(Thyroglobulin)ELISA Kit
Bovine TG(Thyroglobulin)ELISA Kit
| Detection Range | 0.31-20 ng/mL |
| Sensitivity | 0.19 ng/mL |
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T |
| Assay time | 2.5h |
| Detection range | 0.31-20 ng/mL |
| Sensitivity | 0.19 ng/mL |
| Specificity | This kit recognizes Bovine TG in samples. No significant crossreactivity or interference between Bovine TG and analogues was observed |
| Reproducibility | Coefficient of variation is < 10% |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in
this kit has been pre-coated with an antibody specific to Bovine TG. Samples (or
Standards) are added to the micro ELISA plate wells and combined with the specific
antibody. Then a biotinylated detection antibody specific for Bovine TG and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Bovine TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Bovine TG. You can calculate the concentration of Bovine TG in the samples by comparing the OD of the samples to the standard curve.
Components
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate | 8 wells Х 12 strips | 4℃ |
| Reference Standard | 2 vials | 4℃ |
| Reference Standard & Sample Diluent | 1 vials 20mL | 4℃ |
| Micro ELISA Plate | 8 wells Х 12 strips | 4℃ |
| Concentrated Biotinylated Detection Ab | 1 vials 120µL | 4℃ |
| Biotinylated Detection Ab Diluent | 1 vials 10µL | 4℃ |
| Concentrated HRP Conjugate | 1 vials 120µL | 4℃ |
| HRP Conjugate Diluent | 1 vials 10µL | 4℃ |
| Concentrated Wash Buffer（25Х） | 1 vials 30µL | 4℃ |
| Substrate Reagent | 1 vials 10µL | 4℃ |
| Stop Solution | 1 vials 10µL | 4℃ |
| Plate Sealer | 1 vials 10µL | 4℃ |
| Substrate Reagent | 5pieces | 4℃ |
| Manual | 1 copy | 4℃ |
| Desiccant | 1 pack | 4℃ |
| Certificate of Analysis | 1copy | 4℃ |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and
high level Bovine TG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and
high level Bovine TG were tested on 3 different plates, 20 replicates in each plate,
respectively
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.95 | 1.83 | 9.99 | 0.94 | 2.01 | 10.78 |
| Standard deviation | 0.06 | 0.08 | 0.36 | 0.06 | 0.12 | 0.51 |
| CV (%) | 6.09 | 4.62 | 3.57 | 6.59 | 5.78 | 4.77 |
Recovery
The recovery of Bovine TG spiked at three different levels in samples throughout the
range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-101 | 95 |
| EDTA plasma(n=8) | 93-109 | 100 |
| Urine(n=8) | 90-103 | 97 |
Assay Procedures
 |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
 |
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
 |
3. Aspirate and wash the plate for 3 times |
 |
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
 |
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
 |
6. Add 50μL Stop Solution |
 |
7. Read the plate at 450nm immediately. Calculation of the results |