Miniature Shaker KM

Reasons why CO2 incubators require a special shaker

  • Cells in our bodies respire CO2. Local concentrations may get high.
  • Air in COcell culture incubators is about 95% normal air supplemented with 5% COto mimic conditions in our bodies.
  • The bicarbonate anion, HCO3,in our blood helps keep our blood buffered around pH 7.4.
  • Cell culture medium also contains HCO3.
  • Cell culture incubators are humidified. If droplets of water condense on electronics, the water will quickly acidify.

Electronics in CO2

Prolonged storage of your electronic in COincubators will damage them. You will need a CO2 resistant shaker


Miniature Shaker KM CO2 and CO2-FL

The Miniature Shaker KM can tolerate up to 20% CO2. Two models are available. The basic model requires a hole to be placed in the incubator to accommodate the electrical line. The FL model has a flat line that can run between the incubator and the shut door without disturbing the air balance.

Miniature KM Shaker

Two options for powering a shaker operating in a CO2 incubator. The clamps on the unit on the left must be ordered separately.

The clamping strip KM (0052 115) is extra. Only the tray with the rubber mat comes with the shakers. The clamping strip will work with the Miniature Shaker KM Akku as well.

Cells that grow as suspensions in incubators

  • Hybridoma cell lines antibody production
  • Chinese Hamster Ovary Cells (CHO cells) recombinant protein production
  • Human Embryonic Kidney Cells (HEK cells) recombinant protein production

Proper mixing ensures that suspended cells receive proper nutrients.


Miniature Shaker KM 2 Akku

The Akku battery is a trusted source of power in a variety of consumer products. The Battery Shaker KM 2 Akku is the only battery operated shaker currently available on the market. This is a must have shaker for cultures whose shaking cannot be interrupted by intermittent power supplies. Due to this battery attachment, the KM 2 Akku device has great potential for application in field laboratories.

While this unit was not specifically designed for cell culture incubators, the rechargeable battery allowed Appelt-Menzel and coworkers (2017) to test small molecule transport in a blood brain barrier model. Their conditions were 100 rpm, 37°C, 95% humidity, and 5% CO2.

Appelt-Menzel A, Cubukova A, Günther K, Edenhofer F, Piontek J, Krause G, Stüber T, Walles H, Neuhaus W, Metzger M.(2017) Establishment of a Human Blood-Brain Barrier Co-culture Model Mimicking the Neurovascular Unit Using Induced Pluri- and Multipotent Stem Cells. Stem Cell Reports. 8(4):894-906 Free to Download Paper


KM Shaker Accessories

The KM 2 Akku is about the same size as the miniature shakers intended for COincubators. All three have the option of two types of trays that can hold flasks and test tube racks.

KM Accessories

The KM mini universal tray is slightly smaller than the lipped based with the rubber pad. If a spill occurs, liquids flow over the edges of the tray and into the base. In the case of the standard KM, spills (if they occur) flow through the holes in the tray.

The KM series is unique, the basic platform accommodates most petri dishes and tissue culture flasks, which reduces the required number of clamping strip options. The universal tray accommodates most Erlenmeyer flasks used for cell culture. One or more clamping strips may be ordered for the flask. They clamp to the tray that comes with the shaker.

Will KM Shakers work in my laboratory?

 KM CO2 KM CO2-FL KM2 Akku
Motion: orbital orbital orbital
Shaking platform: 200 x 295 mm 200 x 295 mm 200 x 295 mm
Max load: 2 kg 2 kg 2 kg
Shaking speed: 5 - 220 rpm, in steps of 5 5 - 220 rpm, in steps of 5 5 - 220 rpm, in steps of 5
Stroke: 20 mm 20 mm 20 mm
Battery charging time: N/A N/A approx. 10 h
Runtime Battery: N/A N/A min. 40 h
Runtime: 1s - 100 h / continuous 1s - 100 h / continuous 0 - 99 h 59 min / continuous
Order No.: 6136 000 6137 000 6135 000
Electrical supply battery charger: 100 - 240 V, 50 / 60 Hz 100 - 240 V, 50 / 60 Hz 100 - 240 V, 50 / 60 Hz
Internal electrical supply: N/A N/A 1 battery 12 V / 7 Ah
Heat emission: approx. 4 W approx. 4 W approx. 4 W
Ambient temperature: 4°C to 45°C 4°C to 45°C 4°C to 45°C
Relative humidity: 20 - 95% 20 - 95% ~95% (non-condensing)
CO2 concentration: 20.00% 20.00% N/A
Dimensions (w x d x h): 230 x 340 x 145 mm 230 x 340 x 145 mm 250 x 415 x 145 mm
Weight: 7.6 kg 7.6 kg 10.5 kg


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