HIMEDIA RPMI-1640 w/ L-Glutamine, Phenol red, 0.2% Glucose

HIMEDIA RPMI-1640 w/ L-Glutamine, Phenol red, 0.2% Glucose and 0.165 Moles per litre MOPS Buffer w/o Sodium Bicarbonate 10X1L, 20L, 5L

Product Description :

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore et.al for the culture of human normal and neoplastic cells in vitro. RPMI-1640 is the most commonly used medium in the series. A modification of McCoy's 5A medium, the medium was specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium is also known to support growth of cells in the absence of serum. 

AT180 is RPMI-1640 with L-glutamine, phenol red 2gms per litre glucose, 0.165M per litre MOPS buffer. It does not contain sodium bicarbonate. MOPS, a zwitterionic buffer does not antagonize antifungal agents at final concentration of 0.165mol/L for pH 7.0. Therefore, this medium is used as a diluent for antifungal agents that are water-soluble as well as water-insoluble. For water-insoluble antifungal agents, that cannot be prepared as stock solutions in water, such as amphotericin B, anidulafungin, itraconazole, ketoconazole, posaconazole and voriconazole, a dilution series of the agent should be prepared first at 100 times final strength in an appropriate solvent. Each of these non-aqueous solutions should then be diluted tenfold in RPMI-1640 broth". Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. Adapted from Clinical and Laboratory Standards Institute document M27-A3 - Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard - Third edition; Vol.28 No.14

Composition :

Directions :

1. Suspend 44.9gms in 900ml tissue culture grade water with constant, gentle stirring until the medium is completely dissolved. Do not heat the water.
2. Add 2.0gms of sodium bicarbonate powder (TC230) or 26.67ml of 7.5% sodium bicarbonate solution (TCL013) for 1 litre of medium and stir until dissolved.
3. Adjust the pH to 0.2-0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration.
4. Make up the final volume to 1000ml with tissue culture grade water.
5. Sterilize the medium immediately by filtering through a sterile membrane filter with porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide.
6. Aseptically add sterile supplements as required and dispense the desired amount of sterile medium into sterile containers.
7. Store liquid medium at 2-8°C and in dark till use.