GAPDH Polyclonal Antibody Reactivity Human
SKU: E-AB-40337-200
GAPDH Polyclonal Antibody Reactivity Human
| SKU # | E-AB-40337 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein |
| Abbre | GAPDH |
| Synonyms | BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, cb609, p38 component, aging associated gene 9 protein |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW | 35 kDa
|
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:3000-1:6000 |
| IHC | 1:300-1:500 |
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.