DnaseⅠPolyclonal Antibody
SKU: E-AB-40592-200
DnaseⅠPolyclonal Antibody
| SKU # | E-AB-40592 |
| Reactivity | Human |
| Host | Rabbit |
| Applications | WB |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human DnaseⅠ protein expressed by E.coli. |
| Abbre | DnaseⅠ |
| Synonyms | DNASE1, DNL1, DRNI, Deoxyribonuclease-1 |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW | 31 kDa |
| Cellular Localization | Secreted, Zymogengranule, Nucleusenvelope. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:1000-2000 |
Background
Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave DNA in the extracellular space down to an average of tetranucleotides with 5′ monophosphate and 3′ hydroxyl DNA ends . Both single-stranded DNA and double-stranded DNA are degraded by Dnase I. This nuclease appears to account for the major nucleolytic activity on DNA in serum and is responsible for the degradation of the majority of circulating DNA derived from apoptotic and necrotic cell death and from neutrophil extracellular traps. In addition to its role in the serum, it has been proposed as one of the deoxyribonucleases responsible for DNA fragmentation in the process of apoptosis.