Avi-Tag Polyclonal Antibody
SKU: E-AB-40509-200
Avi-Tag Polyclonal Antibody
| SKU # | E-AB-40509 |
| Reactivity | All |
| Host | Rabbit |
| Applications | WB |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | All |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide corresponding to AVI tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Avi-Tag |
| Synonyms | Avi-Tag |
| Calculated MW | 20 kDa |
| Observed MW | 20 kDa |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:2000-5000 |
Background
Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. Biotinylation of the Avi-tag is enzymatically added by the E. coli biotin ligase BirA. This results in a single biotin molecule covalently attached to the lysine residue within the Avi-tag, allowing for highly specific and strong binding to streptavidin or avidin-coated surfaces. This tight and stable interaction is useful for a variety of applications including protein purification, immobilization, and detection in assays such as Western blotting, ELISA, and surface plasmon resonance. The site-specific nature of biotinylation ensures uniformity and reduces potential interference with protein function, making the Avi-tag a valuable tool in protein engineering and biochemical studies.