MSE PRO™ Rat TFAM(Transcription Factor A, Mitochondrial) ELISA Kit

Product name	Rat TFAM(Transcription Factor A, Mitochondrial) ELISA Kit
Reactivity	Rat
Alternative Names	MtTF1; TCF6; TCF6L2; mtTFA; Mitochondrial transcription factor 1; Transcription factor 6-like 2
Assay Type	Sandwich
Sensitivity	0.11 ng/mL
Standard	20 ng/mL
Detection Range	0.32-20 ng/mL
Sample Type	serum, plasma and other biological fluids
Assay Length	3.5h
Research Area	Signal transduction
Uniprot ID	Q91ZW1
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFAM in the samples is then determined by comparing the OD of the samples to the standard curve.

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