MSE PRO™ Cell Fusion System for Electrofusion/Electroporation

The MSE PRO™ Cell Fusion System utilizes pulses of electric fields that can induce the fusion of neighboring cells (electrofusion) or create a porous cell membrane (electroporation) to allow for transfection of macromolecules. Both techniques are reproducible and non-toxic techniques widely used in researching cell-cell interactions and intracellular pathways. Many applications have clinical significance in diagnostic testing, therapeutics and vaccine development.

Offered in 4 different electrode spacings and volume inputs

SKU# MA1005: 0.5mm Electrode Spacing, 20μL volume input
SKU# MA1006: 1.0mm Electrode Spacing, 40μL volume input
SKU# MA1007: 2.0mm Electrode Spacing, 80μL volume input
SKU# MA1008: 3.2mm Electrode Spacing, 700μL volume input

Applications

Stem cell development
Nuclear transfer for transgenic development
Production of hybridomas (fusion of B-cells and Myeloma cells) for antibody expression
Genetically modified plant protoplast, tetraploid fusion
Embryo manipulation
Live gene/drug introduction

Specifications

SKU #	MA1005	MA1006	MA1007	MA1008
Electrode Spacing (mm)	0.5	1.0	2.0	3.2
Sample Input Volume (μL)	20	40	80	700
Dimensions (LxWxH, mm)	450x370x290	450x370x290	450x370x290	450x370x290
Power Source (V/Hz)	110/60	110/60	110/60	110/60

Performance Descriptions

For maintenance of the machine, simply clean frequently using a mild cleaning agent and then disinfect with Ethanol.

Performance	Description
String AC electric field frequency 1MHz-2.5MHz (optimal stable value)	Biological cells can be arranged in strings in an alternating electric field and come into contact with each other. This frequency is designed for a wide range to provide the best frequency choice for the arrangement and fusion of different types of cells or protoplasts of animals, plants, and microorganisms
String AC electric field voltage 1-58V	This voltage range can collect cells ranging from plant protoplasts to bacterial volumes. This voltage range also meets the voltage needs of large volume fusion chambers with wide electrode spacing.
Fusion pulse voltage range 5-600V	This voltage range allows biological cells of any size to induce confluence in fusion chambers at different pole distances.
Gate time 10-1000US	The AC field can be turned off at a set time when a fusion pulse is applied. This is equivalent to inserting a DC pulse into the AC pulse.
Pulse width 5-5000US	Precisely control the timing of fusion pulses. Inducing fusion with multiple short pulses yields higher cell viability than confluence with a single long pulse.
Pulses 1-9	Adjusting the number of fusion pulses applied adds a selection index to determine the optimal fusion conditions for biological cells.

Alternating Electrical Field Parameters

Square Wave Series Pulse Voltage	Peak-to-peak 1-58V, continuously adjustable
Square Wave Series Pulse Frequency	30KH-3000KHz, continuously adjustable
Gate time	10-1000μs, adjustable in 1μs increments, continuously adjustable

Direct Current Electrical Field Parameters

Square Wave Fusion Pulse Voltage	5-600V, continuously adjustable, field strength up to 6000V/cm with electrode spacing of 1.0mm
Square Wave Fusion Pulse Width	5-600V, continuously adjustable, field strength up to 6000V/cm with electrode spacing of 1.0mm
Square Wave Fusion Pulse Cycles	1-9
Randomly Matched Plate Electrodes:	Electrode spacing of 1mm, 2mm
Display Mode	7-inch TFT Touchscreen
Optional Electrodes	Microscope Slide