MSE PRO™ Mouse IL6(Interleukin 6) ELISA Kit

Product name	Mouse IL6(Interleukin 6) ELISA Kit
Reactivity	Mouse
Alternative Names	IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Assay Type	Sandwich
Sensitivity	3.2 pg/mL
Standard	500 pg/mL
Detection Range	7.82-500 pg/mL
Sample type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3.5h
Research Area	Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology
Uniprot ID	P08505
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

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