ATTO WSL-1565 Kronos HT-Bioluminescence measurement system for living cell and tissues

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The Kronos HT is performing to detect high sensitivity for dim and weak luminescence consecutively in culture samples such as cells and tissues in 24 well plates for a long time. 96 well plates and 35 mm dishes also applicable.

Features

Sample stage is in the incubation chamber in which temperature and CO2 gas concentration is controlled and humidity is kept >90%RH.
24・96 wells micro plate applicable.
Long-time detection available *1hour～30days
2 plates are measured by 2 PMTs (one PMT to one plate)
Plates are not moved, PMTs are moved to each well. > No shaking cells, so less damage to delicate cells and applicable to floating cells such as hematocytes.
PMTs are cooled to 10℃ > The dark noise is lower.
Built in filters for Multi-color luciferase assay

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Technical Data:

Model	WSL-1565
Detector	PhotoMultiplier Tube (PMT) (10℃ cooling) x 2 units
Measurement	Photon counting method
Measurement time	1 - 60s/well, for 1 h - 30 days
Format	24 well clear bottom plate x 2 (standard) 96 well clear bottom plate x 2 (optional) 35 mm dish x 12 (optional)
Detection plate	2 PMTs (one PMT to one plate)
Number of specimens	48 samples (24 well plate x 2) standard 192 samples (96 well plate x 2) optional 12 samples (35 mm dish x 12) optional
Incubation temperature control	Room temperature +5°C~45°C (0.1°C steps) in a room temperature environment of 20~28°C
CO₂ gas control	Adjust CO2 gas concentration with CO2 gas mixing unit and introduce it into the cabinet (setting range: 1.0 - 20%)
Humidification	Setting of water receiver in incubator and humidified 5% CO₂ gas flowing intermittently by humidifying out Maintaining > 90% RH
Multi-color assay	Measurement with automatically selected optical filter up to 2 colors
PC requirements	OS: Windows 11, 64/32 bit, Memory: 4GB HDD: more than 20 GB
PC interface	USB 2.0 x 1 port
Dimension(mm)	650 (W) x 520 (D) x 340 (H)
Weight (Kg)	40
Power	AC 100~240V 50/60Hz 400W (Max)

Name	CO2 gas inlet unit for Kronos HT
CO2 control	Adjust the concentration in CO2 gas mixing unit and introduce it into the culture tank (setting range: 1.0 - 20%)
Humidification	Introducing CO2 gas into the Kronos HT culture tank via a humidification unit
Dimensions (mm)	CO2 gas mixing unit: 121 (W) × 174 × 157 (H) Humidification unit: 170 (W) × 140 (D) × 115 (H)
Weight(Kg)	CO2 gas mixing unit: 1.6 Humidification unit: 1.6
Power	CO2 gas mixing unit: AC100~240V, 50/60Hz. 25W Humidification Unit: AC100~240V, 50/60Hz, 30W

Citation and references(Kronos HT (WSL-1565))

(Cytotoxicity) S. Wakuri, K. Yamakage, Y. Kazuki, K. Kazuki, M. Oshimura, S. Aburatani, M. Yasunaga and Y. Nakajima, Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase. Anal. Biochem., 522, 18-29 (2017)[PubMed]
(Oxidative stress) Y. Tabei, K. Murotomi, A. Umeno, M. Horie, Y. Tsujino, B. Masutani, Y. Yoshida, Y. Nakajima, Antioxidant properties of 5-hydroxy-4-phenyl-butenolide via activation of Nrf2/ARE signaling pathway. Food Chem. Toxicol., 107(A), 129-137 (2017)[PubMed]
(Micro RNA) Y. Mie, Y. Hirano, K. Kowata, A. Nakamura, M. Yasunaga, Y. Nakajima and Y. Komatsu, Function control of anti-microRNA oligonucleotides using interstrand cross-linked duplexes, Mol. Ther. Nucleic Acids, 10, 64-74 (2018)[PubMed]
(Anti-inflammation) P. Saiki, Y. Kawano, Y. Nakajima, LJLD. Van Griensven and K. Miyazaki, Novel and stable dual-color IL-6 and IL-10 reporters derived from RAW 264.7 for anti-inflammation screening of natural products. Int. J. Mol. Sci., 20(18), 4620 (2019)[PubMed]
(Cytotoxicity) S. Iwado, S. Abe, M. Oshimura, Y. Kazuki and Y. Nakajima, Bioluminescence measurement of time-dependent dynamic changes of CYP-mediated cytotoxicity in CYP-expressing luminescent HepG2 cells. Int. J. Mol. Sci., 22(6), 2843 (2021)[PubMed]
(Clock gene) JS. Huh, S. Lee, DS. Kim, MS. Choi, H. Choi and KH. Lee, Antioxidative and circadian rhythm regulation effect of Quercus gilva extract. J. Korean Wood Sci. Technol., 50(5), 338-352 (2022)[Link]

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