ATTO AB-2550 Kronos Dio-Real Time Monitoring Gene Expression in Cultured Cells and Tissue Slices

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AB-2550 Chronos Dio is a luminometer that uses a photomultiplier tube as a detector. It is ideal for monitoring gene expression in cultured cells, tissue slices, etc. in real time for several hours to several days using a 35mm diameter culture dish as a sample container.

Purpose and Application

Luciferase reporter assay for real-time gene expression (transcriptional activity) in live cells
Response of cultured cells to drug stimulation

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Technical Data:

Model	AB-2550
Measuring vessel	Cell culture dish 35mm diameter
Number of specimens	8 pieces (8 dishes can be set)
Constant temperature method	Peltier/Air Circulation Method
Chamber temperature setting	(Room temperature -5℃) to 45℃ in 1℃ increments
Temperature accuracy inside the chamber	±0.5℃ (at room temperature of 25℃)
CO2 gas	Controlled by sensor and solenoid valve (concentration 5%)
CO2 _Gas Sensor Accuracy	±0.1%
Detector	Photomultiplier tube
Measurement method	Photon counting method using photomultiplier tubes
Detection Wavelength Range	350nm to 670nm
Luminescence measurement time	1 to 60 seconds/1 to 60 minutes
Filters	F0: None (all light) F1: O56 filter F2: R62 filter
Color separation mechanism	Automatic filter switching mechanism allows separation of up to 3 colors
control	Control software from a PC (Windows 11/10) (up to 5 units can be controlled individually)
Connecting to a PC	USB
Dimensions(mm)	280(W)×400(D)×330(H)
Weight(Kg)	16
power supply	AC100-230V, 50/60Hz, 110W

Citation and References

(Anti-inflammation, IL-6, IL-10) P. Saiki, Y. Nakajima, LJLD. Van Griensven, K. Miyazaki, Real-time monitoring of IL-6 and IL-10 reporter expression for anti-inflammation activity in live RAW 264.7 cells. Biochem. Biophys. Res. Commun., 503(3), 885-890 (2018)[PubMed]
(Clock gene, UV stress) G. Kawamura, M. Hattori, K. Takamatsu, T. Tsukada, Y. Ninomiya, I. Benjamin, P. Sassone-Corsi, T. Ozawa and T. Tamaru, Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress. Commun. Biol., 1, 204 (2018)[PubMed]
(Clock gene) AR. Saran, D. Kalinowska, S. Oh, R. Janknecht, L. DiTacchio, JMJD5 links CRY1 function and proteasomal degradation. PLoS Biol., 16(11), e2006145 (2018)[PubMed]
(Clock gene) J. Lee, E. Park, GH. Kim, I. Kwon and K. Kim, A splice variant of human Bmal1 acts as a negative regulator of the molecular circadian clock. Exp. Mol. Med., 50(12), 159 (2018)[PudMed]
(Clock gene) Y. Maruyama, Y. Asaoka, K. Nakahama, T. Tamaru, K. Takamatsu, N. Takamatsu, A. Hattori, S. Nishina, N. Azuma, A. Kawahara, K. Kume and H. Nishina, The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish. Sci. Rep., 9(1), 196 (2019)[PubMed]
(Clock gene, ER stress) L. Gao, H. Chen, C. Li, Y. Xiao, D. Yang, M. Zhang, D. Zhou, W. Liu, A. Wang and Y. Jin, ER stress activation impairs the expression of circadian clock and clock-controlled genes in NIH3T3 cells via an ATF4-dependent mechanism. Cell. Signal., 57, 89-101 (2019)[PubMed]

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