Tribioscience β-Glucuronidase Assay (TBS2110)

Tribioscience β-Glucuronidase Assay (TBS2110)

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β-Glucuronidases are hydrolytic enzymes responsible for the breakdown of carbohydrates. Specifically, β-Glucuronidases cleave the terminal β-D-glucuronic acid residue from the nonreducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Glucuronidase activity results in metabolic disease and health problems. It is important to detect β-Glucuronidase activity in the tested samples for disease examination.

The Beta-Glucuronidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring glucuronidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of glucuronidase activity in a variety of samples.

Applications

This kit is used for the determination of β-Glucuronidases activity in biological samples.

Key features

• Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30 minutes reaction at 37˚C.
• High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

For Research Use only

Preparation

Standard Curve