Recombinant Rat Tie2/TEK Protein (Fc Tag)(Active)
SKU: PKSR030377-200
Recombinant Rat Tie2/TEK Protein (Fc Tag)(Active)
| SKU # | PKSR030377 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Tie-2, Tie2 |
| Species | Rat |
| Expression Host | HEK293 Cells |
| Sequence | Met 4-Leu 743 |
| Accession | NP_001099207.1 |
| Calculated Molecular Weight | 108 kDa |
| Observed Molecular Weight | 120-130 kDa |
| Tag | C-hFc |
| Bio-activity | Immobilized S1h-3C-mANGPT2 at 10 μg/mL (100 μL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62μg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.