Recombinant Human IL-12 p40 protein(N-His)(active)
SKU: PKSH034191-100
Recombinant Human IL-12 p40 protein(N-His)(active)
| SKU # | PKSH034191 |
| Expression Host | E.coli |
Description
| Synonyms | IL-12/IL-23 p, IL-12 subunit p, NK cell stimulatory factor chain, NK cell Stimulating Factor Chain, CLMF p, IMD, IL12B, CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40), IL-12/IL-23 p40, NK cell Stimulating Factor Chain 2, p40, Interleukin-12 subunit beta, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, IL 12B, IL12 p40, Interleukin 12 beta chain, Interleukin 12 p40 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ile 23-Ser 328 |
| Accession | P29460 |
| Calculated Molecular Weight | 35.6 kDa |
| Observed Molecular Weight | 45 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce cell proliferation in PHA-activated human peripheral blood lymphocytes (PBMC) using a concentration range of 5-50 ng/mL. Note: Results may vary from different PBMC donors. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-12 subunit beta (IL-12B) belongs to the type I cytokine receptor family. It contains 1 fibronectin type-III domain and 1 Ig-like C2-type domain. IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor encoded by IL12B and a 35 kD subunit encoded by IL12A. IL12 is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.