HIF1AN Polyclonal Antibody
SKU: E-AB-13556-200
HIF1AN Polyclonal Antibody
| SKU # | E-AB-13556 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human HIF1AN |
| Abbre | HIF1AN |
| Synonyms | DKFZp762F1811, FIH 1, FIH-1, FIH1, FLJ20615, FLJ22027 , Factor inhibiting HIF-1, Factor inhibiting HIF1, HIF1AN, HIF1N, Hyp, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor, Hypoxia inducible factor asparagine hydroxylase |
| Swissprot | |
| Calculated MW | 40 kDa |
| Cellular Localization | Nucleus. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:50-1:300 |
Background
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha.Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha.This hydroxylation event blocks the association of HIF-1alpha with co-activators.FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha.In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.