ATTO AB-1850 LumiFl SpectroCapture

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Purpose and Application:

LumiFLspectrocapture is a highly sensitive spectrophotometer that can measure the spectrum of weak light from bioluminescence or chemiluminescence, which was difficult to measure with existing spectrophotometers using photodiodes or PMT. LumiFLspectrocapture can acquire a whole range of light (ranging from 400-800nm) simultaneously with an ultrasensitive cooled CCD. It can be a powerful tool in designing bioluminescent/chemiluminescent probes in cell imaging study or analysis of mechanisms of bioluminescent/chemiluminescent reaction.

Features:

An electronic cooled CCD camera is used as a detector.
A CCD is capable of receiving the light of the entire target wavelength region for measurement at one time and detecting it.
A CCD has higher quantum efficiency than a photomultiplier tube.
detection time is shortened with all wavelength simultaneous detection.
Measurement wavelength range is 400 to 800nm.
Long-time exposure is available (high sensitive detection of micro specimens)
When a fluorescent excitation light source is equipped, FRET or fluorescent substance can be measured.

MSE Supplies is an authorized distributor of ATTO products.

Technical Data:

Model and name	AB-1850 Lumiful Spectro Capture
1. Luminescence fluorescence measurement unit
F-number	F3.0
Measurement wavelength range	400～900nm
Inverse variance	60nm/mm
Wavelength resolution	1.8nm
Reproducibility	±0.6nm
Slit width	0.01mm~5mm(11 steps)
Exposure time	1/30 sec. ~ 60 min (26 steps)
Photosensitive unit	Electronically cooled CCD (-40°C)
2. Spectrometer section
Number of diffraction gratings	1 (standard) Diffraction grating: 150 lines/mm
Blaise wavelength	500nm
3. Measurement sample section
Measuring vessel	Quartz Cell (10mmx10mm) / 0.2m LPCR Tube / 35mm Dish
Sample volume	10μL～100μL
Reagent injection section for instantaneous luminescence	Yes (septum)
Excitation light introduction section	FC connector for incident (standard)
4. Software
function	Measurement Control and Spectral Data Analysis
interface	USB2.0
5. Dimensions and Weight
Dimensions & Weight	510mm(W)×725mm(D)×390mm(H), 58kg
power	AC100V, 50/60Hz, 290VA

Citation and References:

Y. Mizui, M. Eguchi, M. Tanaka, Y. Ikeda, H. Yoshimura, T. Ozawa, D. Citterio and Y. Hiruta, Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues. Org. Biomol. Chem., 19(3), 579-586 (2021)[PubMed]
S. Tamaki, N. Kitada, M. Kiyama, R. Fujii, T. Hirano, SB. Kim and S. Maki, Color‑tunable bioluminescence imaging portfolio for cell imaging. Sci. Rep., 11(1), 2219 (2021)[PubMed]
JW. He, GC. Liu, PX. Dong, ZW. Dong, RP. Zhao, W. Wang and XY. Li, Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes). Photocem. Photobiol. Sci., 20(8), 1053-1067 (2021)[PubMed]
K. Teranishi, Non-invasive and accurate readout of superoxide anion in biological systems by near-infrared light. Anal. Chim. Acta., 1179, 338827 (2021)[PubMed]
S. Kanie, D. Miura, N. Jimi, T. Hayashi, K. Nakamura, M. Sakata, K. Ogoh, Y. Ohmiya and Y. Mitani, Violet bioluminescent Polycirrus sp. (Annelida: Terebelliformia) discovered in the shallow coastal waters of the Noto Peninsula in Japan. Sci. Rep., 11(1), 19097 (2021)[PubMed]
E. Belleti, VR. Bevilaqua, AMM. Brito, DA. Modesto, AJC. Lanfredi, VR. Viviani and IL. Nantes‑Cardoso, Synthesis of bioluminescent gold nanoparticle–luciferase hybrid systems for technological applications. Photochem. Photobiol. Sci., 20(11), 1439-1453 (2021)[PubMed]
Y. Ikeda, M. Orioka, T. Nomoto, Y. Hiruta, N. Nishiyama and D. Citterio, Differential Effect of azetidine substitution in firefly luciferin analogues. Chembiochem, 22(21), 3067-3074 (2021)[PubMed]

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