Sucrose Fluorometric Assay Kit

SKU: E-BC-F042

$ 297⁹⁵
Save $ 53⁰⁰

Sucrose Fluorometric Assay Kit

SKU #	E-BC-F042
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
Detection Method	Fluorescence method

Product Details

Properties

Sample Type	Plant tissue
Sensitivity	0.15 μmol/L
Detection Range	0.15-15 μmol/L
Detection Method	Fluorescence method
Assay Time	70 min
Precision	inter-assay CV: 6.5% \| intra-assay CV: 2.3%
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid period	12 months

Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Corn tissue homogenate	1500
10% Potato tissue homogenate	300-500
10% Tomato tissue homogenate	200-300
10% Macrophanerophytes leaf tissue homogenate	200-400
10% Carrot tissue homogenate	1500
10% Onion tissue homogenate	500-1000
10% Green pepper tissue homogenate	500-1000
10% Bush leaves tissue homogenate	20-50

The diluent is extraction working solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.

Detection Principle

Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Kit Components & Storage

Item	Component	Size (96 T)	Storage
Reagent 1	Extraction Solution	45 mL × 1 vial	-20℃, 12 months
Reagent 2	Enzyme Reagent 1	Powder × 1 vial	-20℃, 12 months shading light
Reagent 3	Buffer Solution	10 mL × 1 vial	-20℃, 12 months
Reagent 4	Enzyme Reagent 2	Powder × 1 vial	-20℃, 12 months shading light
Reagent 5	Probe	0.25 mL × 1 vial	-20℃, 12 months shading light
Reagent 6	Standard	Powder × 1 vial	-20℃, 12 months
	Black Microplate	96 wells	No requirement
	Plate Sealer	2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three tomato tissue samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters	Sample 1	Sample 2	Sample 3
Mean (μmol/L)	1.00	7.00	13.00
%CV	2.5	2.2	2.2

Inter-assay Precision

Three tomato tissue samples were assayed 20 times in duplicate by three operators to determine precision between assays

Parameters	Sample 1	Sample 2	Sample 3
Mean (μmol/L)	1.00	7.00	13.00
%CV	6.4	6.8	6.3

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 96%.

	Standard 1	Standard 2	Standard 3
Expected Conc. (μmol/L)	1.5	7.5	10
Observed Conc. (μmol/L)	1.5	7.1	9.5
Recovery rate (%)	98	95	9.5

Sensitivity

The analytical sensitivity of the assay is 0.15 μmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve:

As the fluorescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (μmol/L)	0	1	2	5	8	10	12	15
Fluorescence value	3020	4234	4953	8142	11568	13374	15512	18765
Fluorescence value	3130	4268	5212	8319	11609	13484	15714	18776
Average fluorescence value	3075	4251	5083	8231	11589	13429	15613	18771
Absoluted fluorescence value	0	1176	2008	5156	8514	10354	12538	15696