Xanthine Oxidase (XOD) Activity Assay Kit

$ 323⁹⁵

Xanthine Oxidase (XOD) Activity Assay Kit

SKU #	E-BC-K805-M
Detection Instrument	Microplate reader (540-560 nm, optimum wavelength: 550 nm)
Detection method:	Colorimetric method

Product Details

Properties

Synonyms	XOD
Sample type	Serum, plasma and animal tissue samples
Sensitivity	0.067 U/L
Detection range	0.067-39.30 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	35 min
Precision	Average inter-assay CV: 9.900% \| Average intra-assay CV: 3%
Other instruments required	Incubator(37℃)
Other reagents required	Normal saline (0.9% NaCl)
Storage	-20℃
Valid period	12 months

Dilution of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rat plasma	1
Rat serum	1
10% Mouse kidney tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Mouse liver tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Detection Principle

XOD can catalyze hypoxanthine to xanthine, and at the same time while producing superoxide anion free radicals. In the presence of electron acceptor and chromogenic agent, the purplish red substance can be generated, and the vitality of XOD can be calculated by measuring the production of purplish red substance.

Kit Components & Storage

Item	Component	Size 1(48 T)	Size 2(96 T)	Storage
Reagent 1	Buffer Solution	10 mL × 1 vial	20 mL × 1 vial	-20℃, 12 months
Reagent 2	Substrate Solution	0.5 mL × 1 vial	1 mL × 1 vial	-20℃, 12 months, shading light
Reagent 3	Enzymatic Reagent	0.5 mL × 1 vial	1 mL × 1 vial	-20℃, 12 months, shading light
Reagent 4	Chromogenic Agent 1	0.8 mL × 1 vial	1.6 mL × 1 vial	-20℃, 12 months, shading light
Reagent 5	Chromogenic Agent 2	0.8 mL × 1 vial	1.6 mL × 1 vial	-20℃, 12 months, shading light
Reagent 6	1 mmol/L Standard Solution	1.6 mL × 1 vial	3.2 mL × 1 vial	-20℃, 12 months
	Microplate	96 wells		No requirement
	Plate Sealer	2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	1.20	13.50	26.40
%CV	3.4	3.0	2.6

Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters	Sample 1	Sample 2	Sample 3
Mean (U/L)	1.20	13.50	26.40
%CV	9.0	10.4	10.3

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 99%.

	Standard 1	Standard 2	Standard 3
Expected Conc. (mmol/L)	0.25	0.46	0.77
Observed Conc. (mmol/L)	0.2	0.4	0.9
Recovery rate(%)	89	93	115

Sensitivity

The analytical sensitivity of the assay is 0.067 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (mmol/L)	0	0.2	0.3	0.4	0.6	0.7	0.8	1
OD value	0.042	0.194	0.268	0.327	0.484	0.561	0.631	0.757
OD value	0.041	0.192	0.263	0.350	0.474	0.550	0.616	0.743
Average OD	0.042	0.193	0.266	0.339	0.479	0.556	0.624	0.750
Absoluted OD	0	0.152	0.224	0.297	0.438	0.514	0.582	0.709