Tyrosine Ammonia-lyase (TAL) Activity Assay Kit
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Tyrosine Ammonia-lyase (TAL) Activity Assay Kit
| SKU # | E-BC-K851-M |
| Detection Instrument | Microplate reader (323-343 nm, optimum wavelength: 333 nm) |
|
Detection method |
Colorimetric method |
Product Details
Properties
| Synonyms |
TAL |
| Sample type | Fruit juices, plant and animal tissue |
| Sensitivity | 0.12 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Other instruments required | Micropipette, Incubator, Centrifuge, Water bath |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 20% Epipremnum aureum tissue homogenate | 1 |
| 20% Celery tissue homogenate | 1 |
| 20% Cilantro tissue homogenate | 1 |
| 20% Rat liver tissue homogenate | 1 |
| 20% Rat kidney tissue homogenate | 1 |
| 20% Corn tissue homogenate | 1 |
Note: The diluent is reagent 1.
Detection Principle
TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Extracting Solution | 25 mL × 1 vial | 50 mL × 1 vial | 2-8℃, 12 months |
| Reagent 2 | Buffer Solution | 35 mL × 1 vial | 35 mL × 2 vials | 2-8℃, 12 months |
| Reagent 3 | Substrate | Power ×1 vial | Power ×2 vials | 2-8℃, 12 months |
| Reagent 4 | Stop Solution | 1.5 mL × 1 vial | 1.5 mL × 2 vials | 2-8℃, 12 months |
| UV Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Sensitivity
The analytical sensitivity of the assay is 0.12 U/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.