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The Fast Genotyping system is designed for rapid DNA extraction and PCR amplification from zebrafish (TBS4035) tail or fin clip, or other tissue. With this system, the DNA extraction can be done within 10 minutes. The PCR product can be directly loaded onto gel for genotype analysis.
The 2x Genotyping PCR Mix contains all reagents required for PCR including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, tracking dye, density, and PCR stabilizers and enhancers at optimal concentrations for consistent and efficient PCR amplification. All you need is to add primers, the DNA template, and water. The PCR products can be directly loaded onto the electrophoresis gel.
Key Features
- Convenient: The system contains all components for DNA extraction and PCR amplification.
- No Loading Dye Required: The PCR products can be directly loaded onto the electrophoresis gel.
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High Fidelity: The optimal buffer condition and specifically engineered Taq DNA polymerase have increased the efficiency of PCR amplification.
Kit Contents
- Fast DNA Extraction: 10mL
- 2x PCR Ready Mix: 2x 1.25mL
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Sufficient reagent for 250 x 20μL
Storage Conditions
The kit is shipped on ice. Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.