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Tribo™ Reverse Transcription Kit is optimized to synthesize first-strand cDNA for PCR or qPCR. The optimal first-strand cDNA synthesis temperature is 50°C. The reverse transcription reaction at higher temperatures improves cDNA yields for difficult RNA transcription. This pre-mixed 5X concentrated solution includes all necessary components for first-strand synthesis. With the mixture of 5X RT buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, and Reverse Transcriptase, the first-strand cDNA synthesis process is greatly simplified.
Applications
This kit can be used for first-strand cDNA synthesis.
Key Features:
- Easy-to-use pre-mixed package (All in One)
- High fidelity, yield, and sensitivity
- Broader linear range
- Complete cDNA synthesis guaranteed
- Robust and high throughput
- Work with difficult sequences
Kit Contents
5x RT reagent: 0.2mL
The kit contains RT buffer, dNTPs, DTT, RNase inhibitor, Primer mixer, and Reverse Transcriptase. The reagent is enough for 50 RT reactions in 20μL.
Storage Conditions
The kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.