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The 2x PCR Blue Mix is a ready-to-use solution containing all reagents required for PCR including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, tracking dye, density and PCR stabilizers and enhancers at optimal concentrations for consistent and efficient PCR amplification. It simplifies PCR assembly, so that only primers, DNA template and water need to be added. The PCR products can be directly loaded onto the electrophoresis gel.
Applications
- Routine PCR using genomic, viral, and plasmid templates; RT-PCR
- DNA and colony screening; DNA cloning and subcloning
- Genotyping; DNA mutagenesis
Key Features:
- Convenient: The 2x PCR Blue Mix kit contains all components needed for PCR amplification. All you need to do is to add primers, template, and water for PCR reaction.
- Hazard-free Visualization: Dyes are non-toxic and non-mutagenic.
- No Loading Dye Required: The PCR products can be directly loaded onto electrophoresis gel.
- High efficiency: the optimal buffer condition and specifically engineered hot start Taq DNA polymerase have increased the efficiency of PCR amplifications.
Kit Contents
- 2x 1.0 mL of 2x PCR Blue Mix
- 1x 2.0 mL of Nuclease-free water.
- Sufficient reagent for 200 x 20µL
Storage Conditions
The kit is shipped on ice. All components should be stored at -20°C in a non-frost-free freezer.
Shelf life is 12 months after receiving.