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Histone H3 acetylation involves the addition of acetyl groups to lysine residues on histone proteins, which neutralizes their positive charge and relaxes chromatin structure, facilitating gene transcription, which are associated with DNA in chromatin. Histone acetylation is regulated by histone acetyltransferases (HATs), which add acetyl groups, and histone deacetylases (HDACs), which remove them. Histone H3 acetylation is tightly involved in cell cycle regulation, cell proliferation, and apoptosis. Disruption of histone acetylation balance in cancer cells, linked to abnormal transcription factor binding, promotes tumorigenesis, cancer progression, and metastasis.
The Total Histone H3 Acetylation Detection Assay is designed to measure histone H3 acetylation in various mammalian cells, including human and mouse cells, as well as fresh and frozen tissues, cultured adherent cells, and suspension cells. This assay is simple, user-friendly, accurate, and fast. The measurement can be completed in 2.5 hours, with a detection range of 2 µg to 5 ng per well.
Alternative Name: H3ac
Kit Content and Storage Conditions
| PART | 48 ASSAYS | 96 ASSAYS | STORAGE OF OPENED/ RECONSTITUTED |
| Detection Antibody | 5 µL of histone H3 antibody(1000x). | 10 µL of histone H3 antibody(1000x). | –20°C |
| Histone H3 Standard | 10 µL of histone H3 control. | 20 µL of histone H3 control. | –20°C |
| Stop Solution | 3 mL of 2 N sulfuric acid. | 6 mL of 2 N sulfuric acid. | Room temperature away from light |
| Wash Buffer | 10 mL of concentrated solution (10x). | 20 mL of concentrated solution (10x). | May be stored for up to 6 months at 2-8 °C.
|
| Antibody Buffer | 6 ml | 12 ml | |
| Color Developer | 5 ml | 10 ml | |
| 8-Well Sample Strips (with Frame) Precoated with H3 antibody | 6 | 12 |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.