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Tobacco Mosaic Virus (TMV) TaqMan RT-PCR Detection Kit is one step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the detection of TMV infection in plant samples. It combines both reverse transcription (RT) and TaqMan probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy to use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting TMV.
TMV TaqMan RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probe, internal control, positive control, negative control, and buffer. The TMV target gene is labeled with fluorescence Fam, and internal control is labeled with Hex.
Applications
This kit is used for the detection of tobacco mosaic virus infection in plant samples.
Key Features
- One-step complete qRT-PCR in a single tube.
- Reduce contamination in the operating process.
- Accurate detection and quantification of the target gene.
- 4x RT-PCR mix makes it easier to adjust the sample size in one-tube reaction.
Storage Conditions
Store all components at -20°C in a non-frost-free freezer.
Shelf life is 12 months after recipient.