This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase and uricase.
Tribioscience Purine-nucleoside phosphorylase, Enzyme Activity (TBP0038)
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Tribioscience Purine-nucleoside phosphorylase, Enzyme Activity (TBP0038)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅢ 15U/mg-solid or more
Contaminants: Catalase ≤20%
5′-Nucleosidase ≤1.0×10⁻³%
Adenosine deaminase ≤1.0×10⁻³%
ATPase ≤1.0×10⁻²%
Stabilizers: K-Gluconate, mannitol, EDTA
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 120,000
Isoelectric point: 4.1±0.1
Michaelis constants: 6.4×10⁻⁵M (Inosine), 3.2×10⁻⁴M (Pi)
Inhibitors: p-Chloromercuribenzoate, SDS, Hg⁺⁺, Ag⁺
Optimum pH: 7.5-8.0
Optimum temperature: 65°C
pH Stability: pH 6.0-9.0 (30°C, 16hr)
Thermal stability: below 60°C (pH 7.7, 30min)
Applications