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Tribioscience Proline specific endopeptidase, Enzyme Activity (TBP0040)

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SKU: TBP0040-50U
Regular price $ 331.95

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Tribioscience Proline specific endopeptidase, Enzyme Activity (TBP0040)

Research Use Only. Not intended for human use.

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅠ 5.0U/mg-solid or more

Contaminants: Leucine aminopeptidase ≤1.0×10⁻¹%

Trypsin-like activity ≤1.0×10⁻¹%

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 78,000

Isoelectric point: 9.1

Michaelis constants: 2.5×10⁻⁵M (Z-Gly-Pro-MCA), 1.4×10⁻⁴M (Z-Gly-Pro-2NNap)

Structure: Monomer

Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH₂Cl

Optimum pH: 6.5

Optimum temperature: 37°C (40°C)

pH Stability: 5.5-8.5 (30°C,15hr)

Thermal stability: below 40°C (pH7.0,10min)

Applications

This enzyme is useful for the determination of amino acid sequences of peptides and proteins containing proline residues.